Mitochondrial activity in bMECs. Mito-Tracker Green is a mitochondrial green fluorescent probe, with intensity of green fluorescence reflecting mitochondrial activity. A1, A2, and A3: In the Control group, mitochondria in normal bMECs had strong green fluorescence, indicating good mitochondrial activity. B1, B2, and B3: In the P. ciferrii infection group, there was weak green fluorescence, indicating decreased mitochondrial activity in bMECs. C1, C2, and C3: In the P. bovis infection group, there was weak green fluorescence in bMECs, indicating mitochondrial activities were decreased.