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Table 2 List of primary and secondary antibodies used for immunocytochemistry.

From: Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer

Antibody Antigen Isotype Cat no. Supplier Working dilution
Mouse anti-chicken CD45 (clone AV53) CD45 IgG1 n/a Institute for Animal Health, UK 1:100
Rabbit anti bovine SP-1 Chromogranin A Chromogranin A IgG 20085 Immunostar 1:750
Mouse anti-human E-cadherin (clone 36) E-cadherin IgG2a 36/Ecadherin BD Biosciences 1:50
Phalloidin Rhodamine F-actin   R415 Invitrogen 1:100
Rabbit anti-chicken Lysozyme (polyclonal) Lysozyme C Poly IgG Ab391 Abcam 1:50
Rabbit anti-human Muc5AC (clone 45M1) Muc5AC IgG1 Ab212636 Abcam 1:50
Mouse anti-chicken Villin (clone 1D2C3) Villin IgG1 Sc-58897 Santa Cruz 1:50
Mouse anti-pig Vimentin (clone V9) Vimentin IgG1 Ab8069 Abcam 1:50
Rabbit anti-human ZO1 (polyclonal) ZO1 IgG Ab216880 Abcam 1:100
Mouse anti-human ZO1 (clone A12) ZO1 IgG1 33-9100 Invitrogen 1:100
Goat anti mouse Alexafluor® 488 Mouse IgG1 IgG1 A-21121 Invitrogen 1:200
Goat anti mouse Alexafluor® 488 Mouse IgG IgG A-11001 Invitrogen 1:200
Goat anti mouse Alexafluor® 546 Mouse IgG2a IgG2a A-21133 Invitrogen 1:200
Goat anti rabbit Alexafluor® 568 Rabbit IgG IgG A-11036 Invitrogen 1:200
Goat anti rabbit Alexafluor® 647 Rabbit IgG IgG A-21244 Invitrogen 1:200