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Table 2 List of primary and secondary antibodies used for immunocytochemistry.

From: Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer

Antibody

Antigen

Isotype

Cat no.

Supplier

Working dilution

Mouse anti-chicken CD45 (clone AV53)

CD45

IgG1

n/a

Institute for Animal Health, UK

1:100

Rabbit anti bovine SP-1 Chromogranin A

Chromogranin A

IgG

20085

Immunostar

1:750

Mouse anti-human E-cadherin (clone 36)

E-cadherin

IgG2a

36/Ecadherin

BD Biosciences

1:50

Phalloidin Rhodamine

F-actin

 

R415

Invitrogen

1:100

Rabbit anti-chicken Lysozyme (polyclonal)

Lysozyme C

Poly IgG

Ab391

Abcam

1:50

Rabbit anti-human Muc5AC (clone 45M1)

Muc5AC

IgG1

Ab212636

Abcam

1:50

Mouse anti-chicken Villin (clone 1D2C3)

Villin

IgG1

Sc-58897

Santa Cruz

1:50

Mouse anti-pig Vimentin (clone V9)

Vimentin

IgG1

Ab8069

Abcam

1:50

Rabbit anti-human ZO1 (polyclonal)

ZO1

IgG

Ab216880

Abcam

1:100

Mouse anti-human ZO1 (clone A12)

ZO1

IgG1

33-9100

Invitrogen

1:100

Goat anti mouse Alexafluor® 488

Mouse IgG1

IgG1

A-21121

Invitrogen

1:200

Goat anti mouse Alexafluor® 488

Mouse IgG

IgG

A-11001

Invitrogen

1:200

Goat anti mouse Alexafluor® 546

Mouse IgG2a

IgG2a

A-21133

Invitrogen

1:200

Goat anti rabbit Alexafluor® 568

Rabbit IgG

IgG

A-11036

Invitrogen

1:200

Goat anti rabbit Alexafluor® 647

Rabbit IgG

IgG

A-21244

Invitrogen

1:200