Table 1 List of reagents used to prepare cell culture media.
Reagent | Concentration | Cat. No | Supplier |
|---|---|---|---|
Basal media | |||
 Advanced DMEM/F12 | 1 ×  | 12634010 | ThermoFisher scientific (TFS) |
 l-glutamine | 2 mM | 25030024 | TFS |
 HEPES | 10 mM | 15630080 | TFS |
 Penicillin/streptomycin | 50 U/mL | TFS | 15140122 |
Seeding media | |||
 Basal media | 1 ×  |  |  |
 B27 supplement | 1 ×  | 17504044 | TFS |
 N2 supplement | 1 ×  | 17502048 | TFS |
 EGF (human) | 100 ng/mL | PHG6045 | TFS |
 LDN 193189 | 100 nM | BV-2092-5 | Cambridge bioscience |
 R-Spondin (human) | 100 ng/mL | 4645-RS | R&D systems |
 CHIR 99021 | 10 µM | S1263-SEL | Stratech scientific |
 Y27632 | 10 µM | 72304 | Stem cell technologies |
Maintenance media | |||
 Basal media | 1 ×  |  |  |
 B27 supplement | 1 ×  |  |  |
 N2 supplement | 1 ×  |  |  |
 EGF (human) | 100 ng/mL |  |  |
 Noggin (human) | 50 ng/mL | 6057_NG_025 | R&D systems |
 R-Spondin (human) | 100 ng/mL |  |  |