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Table 1 List of reagents used to prepare cell culture media.

From: Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer

Reagent

Concentration

Cat. No

Supplier

Basal media

 Advanced DMEM/F12

1 × 

12634010

ThermoFisher scientific (TFS)

 l-glutamine

2 mM

25030024

TFS

 HEPES

10 mM

15630080

TFS

 Penicillin/streptomycin

50 U/mL

TFS

15140122

Seeding media

 Basal media

1 × 

  

 B27 supplement

1 × 

17504044

TFS

 N2 supplement

1 × 

17502048

TFS

 EGF (human)

100 ng/mL

PHG6045

TFS

 LDN 193189

100 nM

BV-2092-5

Cambridge bioscience

 R-Spondin (human)

100 ng/mL

4645-RS

R&D systems

 CHIR 99021

10 µM

S1263-SEL

Stratech scientific

 Y27632

10 µM

72304

Stem cell technologies

Maintenance media

 Basal media

1 × 

  

 B27 supplement

1 × 

  

 N2 supplement

1 × 

  

 EGF (human)

100 ng/mL

  

 Noggin (human)

50 ng/mL

6057_NG_025

R&D systems

 R-Spondin (human)

100 ng/mL