|
Reagent
|
Concentration
|
Cat. No
|
Supplier
|
|---|
|
Basal media
|
|
Advanced DMEM/F12
|
1 ×
|
12634010
|
ThermoFisher scientific (TFS)
|
|
l-glutamine
|
2 mM
|
25030024
|
TFS
|
|
HEPES
|
10 mM
|
15630080
|
TFS
|
|
Penicillin/streptomycin
|
50 U/mL
|
TFS
|
15140122
|
|
Seeding media
|
|
Basal media
|
1 ×
| | |
|
B27 supplement
|
1 ×
|
17504044
|
TFS
|
|
N2 supplement
|
1 ×
|
17502048
|
TFS
|
|
EGF (human)
|
100 ng/mL
|
PHG6045
|
TFS
|
|
LDN 193189
|
100 nM
|
BV-2092-5
|
Cambridge bioscience
|
|
R-Spondin (human)
|
100 ng/mL
|
4645-RS
|
R&D systems
|
|
CHIR 99021
|
10 µM
|
S1263-SEL
|
Stratech scientific
|
|
Y27632
|
10 µM
|
72304
|
Stem cell technologies
|
|
Maintenance media
|
|
Basal media
|
1 ×
| | |
|
B27 supplement
|
1 ×
| | |
|
N2 supplement
|
1 ×
| | |
|
EGF (human)
|
100 ng/mL
| | |
|
Noggin (human)
|
50 ng/mL
|
6057_NG_025
|
R&D systems
|
|
R-Spondin (human)
|
100 ng/mL
| | |
