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Table 1 List of reagents used to prepare cell culture media.

From: Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer

Reagent Concentration Cat. No Supplier
Basal media
 Advanced DMEM/F12 1 ×  12634010 ThermoFisher scientific (TFS)
 l-glutamine 2 mM 25030024 TFS
 HEPES 10 mM 15630080 TFS
 Penicillin/streptomycin 50 U/mL TFS 15140122
Seeding media
 Basal media 1 ×    
 B27 supplement 1 ×  17504044 TFS
 N2 supplement 1 ×  17502048 TFS
 EGF (human) 100 ng/mL PHG6045 TFS
 LDN 193189 100 nM BV-2092-5 Cambridge bioscience
 R-Spondin (human) 100 ng/mL 4645-RS R&D systems
 CHIR 99021 10 µM S1263-SEL Stratech scientific
 Y27632 10 µM 72304 Stem cell technologies
Maintenance media
 Basal media 1 ×    
 B27 supplement 1 ×    
 N2 supplement 1 ×    
 EGF (human) 100 ng/mL   
 Noggin (human) 50 ng/mL 6057_NG_025 R&D systems
 R-Spondin (human) 100 ng/mL