Bacterial components induce inflammatory responses in 2D enteroids. Confluent 2D enteroids cultured on uncoated plastic wells were treated on day 5 for 6 h with LTA from Bacillus subtilis or LPS from S. enterica serotype Typhimurium (1 and 10 μg/mL). Inflammatory responses are assessed by qRT-PCR A IL-6 and B IL-8 (CXCLi2) mRNA expression shown as fold change relative to control 2D enteroids. Statistical significance calculated in comparison to control cells and shown as *P < 0.05. Data is the median of four independent experiments and 95% CI.