Table 1 PCR primers.
From: Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida
Primer | Sequence(5’-3’) | Product size (bp) |
|---|---|---|
qseC 5’Arm-F | GACCATGATTACGCCAAGCTTTGATTGCACGTTTGCAGGCA | 350 |
qseC 5’Arm-R | TTTTATATAATTAAGCCATTTCATCATTTT | |
qseC 3’Arm-F | AATGGCTTAATTATATAAAAGGATTTAGAT | 350 |
qseC 3’Arm-R | TTTATCGGTACCCGGGGATCCAGCGAGATTATTCTACACCG | |
dqseC-F | GCAAACAAGTTTACGAGTTC | 1300 |
dqseC-R | CACATTTTCTAACCGGAATTG | |
pUC19-F | GAGCGGATAACAATTTCACAC | 151 |
pUC19-R | ATTTAAGAATACCTTGCCGC | |
KMT1-F | ATCCGCTATTTACCCAGTGG | 460 |
KMT1-R | GCTGTAAACGAACTCGCCAC | |
egfp-F | GACCATGATTACGCCAAGCTTCCGCGCCAACCGATAAAACC | 1111 |
egfp-R | TTTATCGGTACCCGGGGATCCCAATTCGCCCTATAGTGAGT | |
comqseC-F | CTAGTGAATTCTGCAGTCGACATGAAATGGCTTAAGCAAAC | 1374 |
comqseC-R | TGGCCGTCGTTTTACTCTAGATTAAATTTTATTTAATAAAA |