Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Beuckelaere, Lisa; De Visscher, Anneleen; Souza, Fernando Nogueira; Meyer, Evelyne; Haesebrouck, Freddy; Piepers, Sofie; De Vliegher, Sarne

doi:10.1186/s13567-021-01007-8

Veterinary Research

Table 3 Linear mixed regression models for IgG1, IgG2 and IgG2/IgG1 in dry period and early lactation.

From: Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Outcome variable	Predictor variable	Dry period				Early lactation
Outcome variable	Predictor variable	β^a	SE^b	LSM^c	P	β	SE	LSM	P
IgG1 (OD^g/TP^h)	Quarter inoculation status				NS^d,e				NS^d
	Non-inoculated	Ref.^f	–	0.16	–	Ref.	–	0.00	–
	Inoculated and non-colonized	0.10	0.13	0.26	NS	0.14	0.09	0.08	NS
	Inoculated and colonized	0.11	0.12	0.27	NS	0.13	0.09	0.07	NS
	Time of sampling^k				<0.01^d				–
	D-1	Ref.	–	0.00	–	–	–	–	–
	D14	0.42	0.03	0.27	<0.01	–	–	–	–
	D27	0.52	0.04	0.37	<0.01	–	–	–	–
	D41	0.57	0.04	0.43	<0.01	–	–	–	–
	Time of sampling				–				<0.01^d
	C	–	–	–	–	Ref.	–	0.27	–
	L1	–	–	–	–	−0.17	0.03	0.10	<0.01
	L2	–	–	–	–	−0.25	0.03	0.02	<0.01
	L3	–	–	–	–	−0.26	0.03	0.01	<0.01
	L4	–	–	–	–	−0.30	0.03	−0.03	<0.01
	L5	–	–	–	–	−0.34	0.03	−0.07	<0.01
	L6	–	–	–	–	−0.35	0.03	−0.08	<0.01
	Inoculation status x Time of samplingⁱ	–	–	–	< 0.01^d	–	–	–	NS^d
IgG2 (OD/TP)	Quarter inoculation status				NS^d				0.09^d
	Non-inoculated	Ref.	–	0.00	–	Ref.	–	0.00	–
	Inoculated and non-colonized	0.01	0.02	0.00	NS	0.04	0.01	0.03	0.05
	Inoculated and colonized	0.03	0.02	0.02	NS	0.04	0.01	0.03	0.06
	Time of sampling				<0.01^d				–
	D-1	Ref.	–	0.00	–	–	–	–	–
	D14	0.02	0.00	0.01	<0.01	–	–	–	–
	D27	0.02	0.01	0.01	<0.01	–	–	–	–
	D41	0.01	0.01	0.00	0.01	–	–	–	–
	Time of sampling				–				<0.01^d
	C	–	–	–	–	Ref.	–	0.06	–
	L1	–	–	–	–	−0.04	0.00	0.03	<0.01
	L2	–	–	–	–	−0.05	0.00	0.01	<0.01
	L3	–	–	–	–	−0.06	0.00	0.00	<0.01
	L4	–	–	–	–	−0.06	0.00	0.00	<0.01
	L5	–	–	–	–	−0.07	0.00	0.00	<0.01
	L6	–	–	–	–	−0.07	0.00	−0.01	<0.01
	Inoculation status × Time of samplingⁱ	–	–	–	<0.01^d	–	–	–	<0.01^d
IgG2/IgG1	Quarter inoculation status				NS^d				NS^d
	Non-inoculated	Ref.	–	0.00	–	Ref.	–	0.16	–
	Inoculated and non-colonized	0.27	0.48	−0.15	NS	1.54	0.91	1.70	NS
	Inoculated and colonized	0.32	0.44	−0.10	NS	1.49	0.91	1.65	NS
	Time of sampling				<0.01^d				–
	D-1	Ref.	–	1.01	–	–	–	–	–
	D14	−1.11	0.38	−0.09	<0.01	–	–	–	–
	D27	−1.74	0.41	−0.73	<0.01	–	–	–	–
	D41	−2.10	0.45	−1.09	<0.01	–	–	–	–
	Time of sampling				–				0.01^d
	C	–	–	–	–	Ref.	–	1.47	–
	L1	–	–	–	–	−0.03	0.20	1.45	NS
	L2	–	–	–	–	−0.10	0.21	1.38	NS
	L3	–	–	–	–	−0.39	0.22	1.08	0.07
	L4	–	–	–	–	−0.36	0.22	1.11	0.10
	L5	–	–	–	–	−0.50	0.22	0.98	0.02
	L6	–	–	–	–	−0.74	0.21	0.74	<0.01
	Inoculation status × Time of sampling^j	–	–	–	NS^d	–	–	–	<0.01^d

Linear mixed regression models for the 2-step approach transformed [45] IgG1, IgG2 and IgG2/IgG1 levels [OD/total protein (µg/µL)] in both the dry period and in early lactation
^aRegression coefficient of difference of LSM
^bStandard error
^cLeast square means
^dP value for overall effect
^eNot significant
^fReference
^gOptical density
^hTotal protein concentration
ⁱInteraction term visualized in Figure 5 based on the least square means
^jInteraction term visualized in Figure 6 based on the least square means
^kOverview of the experimental set-up and days of sampling is visualized in Figure 1

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