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Table 3 Linear mixed regression models for IgG1, IgG2 and IgG2/IgG1 in dry period and early lactation.

From: Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Outcome variable Predictor variable Dry period Early lactation
βa SEb LSMc P β SE LSM P
IgG1 (ODg/TPh) Quarter inoculation status     NSd,e     NSd
   Non-inoculated Ref.f 0.16 Ref. 0.00
   Inoculated and non-colonized 0.10 0.13 0.26 NS 0.14 0.09 0.08 NS
   Inoculated and colonized 0.11 0.12 0.27 NS 0.13 0.09 0.07 NS
  Time of samplingk      <0.01d    
   D-1 Ref. 0.00
   D14 0.42 0.03 0.27  <0.01
   D27 0.52 0.04 0.37  <0.01
   D41 0.57 0.04 0.43  <0.01
  Time of sampling         <0.01d
   C Ref. 0.27
   L1 −0.17 0.03 0.10  <0.01
   L2 −0.25 0.03 0.02  <0.01
   L3 −0.26 0.03 0.01  <0.01
   L4 −0.30 0.03 −0.03  <0.01
   L5 −0.34 0.03 −0.07  <0.01
   L6 −0.35 0.03 −0.08  <0.01
  Inoculation status x Time of samplingi  < 0.01d NSd
IgG2 (OD/TP) Quarter inoculation status     NSd     0.09d
   Non-inoculated Ref. 0.00 Ref. 0.00
   Inoculated and non-colonized 0.01 0.02 0.00 NS 0.04 0.01 0.03 0.05
   Inoculated and colonized 0.03 0.02 0.02 NS 0.04 0.01 0.03 0.06
  Time of sampling      <0.01d    
   D-1 Ref. 0.00
   D14 0.02 0.00 0.01  <0.01
   D27 0.02 0.01 0.01  <0.01
   D41 0.01 0.01 0.00 0.01
  Time of sampling         <0.01d
   C Ref. 0.06
   L1 −0.04 0.00 0.03  <0.01
   L2 −0.05 0.00 0.01  <0.01
   L3 −0.06 0.00 0.00  <0.01
   L4 −0.06 0.00 0.00  <0.01
   L5 −0.07 0.00 0.00  <0.01
   L6 −0.07 0.00 −0.01  <0.01
  Inoculation status × Time of samplingi  <0.01d  <0.01d
IgG2/IgG1 Quarter inoculation status     NSd     NSd
   Non-inoculated Ref. 0.00 Ref. 0.16
   Inoculated and non-colonized 0.27 0.48 −0.15 NS 1.54 0.91 1.70 NS
   Inoculated and colonized 0.32 0.44 −0.10 NS 1.49 0.91 1.65 NS
  Time of sampling      <0.01d    
   D-1 Ref. 1.01
   D14 −1.11 0.38 −0.09  <0.01
   D27 −1.74 0.41 −0.73  <0.01
   D41 −2.10 0.45 −1.09  <0.01
  Time of sampling        0.01d
   C Ref. 1.47
   L1 −0.03 0.20 1.45 NS
   L2 −0.10 0.21 1.38 NS
   L3 −0.39 0.22 1.08 0.07
   L4 −0.36 0.22 1.11 0.10
   L5 −0.50 0.22 0.98 0.02
   L6 −0.74 0.21 0.74  <0.01
  Inoculation status × Time of samplingj NSd  <0.01d
  1. Linear mixed regression models for the 2-step approach transformed [45] IgG1, IgG2 and IgG2/IgG1 levels [OD/total protein (µg/µL)] in both the dry period and in early lactation
  2. aRegression coefficient of difference of LSM
  3. bStandard error
  4. cLeast square means
  5. dP value for overall effect
  6. eNot significant
  7. fReference
  8. gOptical density
  9. hTotal protein concentration
  10. iInteraction term visualized in Figure 5 based on the least square means
  11. jInteraction term visualized in Figure 6 based on the least square means
  12. kOverview of the experimental set-up and days of sampling is visualized in Figure 1