Skip to main content

Table 2 Linear mixed regression models for IL-6, IFN-γ and IL-10 in dry period and early lactation.

From: Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Outcome variable Predictor variable Dry period Early lactation
βa SEb LSMc P β SE LSM P
IL-6 (pg/mL) Quarter inoculation status     0.04d     NSd,e
   Non-inoculated Ref.f 133.46 Ref. 34.37
   Inoculated and non-colonized −58.50 17.98 74.96 0.05 −10.90 10.39 23.47 NS
   Inoculated and colonized −75.44 17.49 58.02 0.03 −8.82 10.40 25.55 NS
  Time of samplingh      <0.01d    
   D-1 Ref. 0.00
   D14 109.38 7.37 102.49  <0.01
   D27 140.00 7.52 133.11  <0.01
   D41 133.42 7.97 126.54  <0.01
  Time of sampling         <0.01d
   C Ref. 110.30
   L1 −32.89 6.45 77.41  <0.01
   L2 −83.81 7.67 26.49  <0.01
   L3 −108.58 8.09 1.72  <0.01
   L4 −117.24 8.22 −6.94  <0.01
   L5 −117.35 8.18 −7.05  <0.01
   L6 −117.64 7.80 −7.34  <0.01
  Inoculation status × Time of samplingg  <0.01d  <0.01d
IFN-γ (pg/mL) Quarter inoculation status     NSd     NSd
   Non-inoculated Ref. 0.00 Ref. 0.32
   Inoculated and non-colonized 0.64 0.24 0.64 0.09 0.41 0.21 0.73 0.06
   Inoculated and colonized 0.83 0.22 0.83 0.06 0.19 0.21 0.52 NS
  Time of sampling      <0.01d    
   D-1 Ref. 0.00
   D14 0.21 0.14 0.21 NS
   D27 0.46 0.15 0.46  <0.01
   D41 1.28 0.16 1.28  <0.01
  Time of sampling         <0.01d
   C Ref. 1.43
   L1 −0.27 0.20 1.15 NS
   L2 −0.91 0.23 0.52  <0.01
   L3 −1.25 0.23 0.18  <0.01
   L4 −1.36 0.23 0.06  <0.01
   L5 −1.31 0.23 0.11  <0.01
   L6 −1.21 0.22 0.21  <0.01
  Inoculation status × Time of samplingg  <0.01d  <0.01d
IL-10 (pg/mL) Quarter inoculation status      <0.01d     NSd
   Non-inoculated Ref. 4559.23 Ref. 1361.63
   Inoculated and non-colonized −2618.91 336.24 1940.32  <0.01 −874.42 572.75 487.21 NS
   Inoculated and colonized −1965.67 303.55 2593.56  <0.01 −761.25 573.07 600.38 NS
  Time of sampling      <0.01d    
   D-1 Ref. 0.00
   D14 3832.82 264.36 3740.15  <0.01
   D27 4228.92 285.91 4136.26  <0.01
   D41 4433.06 287.82 4340.40  <0.01
  Time of sampling         <0.01d
   C Ref. 2499.53
   L1 103.34 218.62 2602.87 NS
   L2 −1019.34 262.75 1480.19  <0.01
   L3 −2060.8 278.83 438.73  <0.01
   L4 −3116.52 284.48 −616.99  <0.01
   L5 −2971.28 283.08 −471.75  <0.01
   L6 −2717.27 269.54 −217.74  <0.01
  Inoculation status × Time of samplingg  <0.01d  <0.01d
  1. Linear mixed regression models for the log10 transformed IFN-γ concentration (Log pg/mL) and 2-step approach transformed [45] IL-6 and IL-10 concentrations (pg/mL) in both the dry period and in early lactation
  2. aRegression coefficient of difference of LSM
  3. bStandard error
  4. cLeast square means
  5. dP value for overall effect
  6. fReference
  7. eNot significant
  8. gInteraction term visualized in Figure 4 based on the least square means
  9. hOverview of the experimental set-up and days of sampling is visualized in Figure 1