Figure 6From: Heat shock protein 70 could enhance porcine epidemic diarrhoea virus replication by interacting with membrane proteinsInteraction between the PEDV M protein and HSP70. Cells were transfected with PEDV structure proteins. After 48Â h, the cells were harvested, and immunoprecipitation was performed. The transfected protein molecular weights were as follows: PEDV E (13Â kDa), PEDV M (28Â kDa), PEDV N (55Â kDa) and PEDV S (150Â kDa). A Immunoprecipitation assays demonstrated that endogenous HSP70 bound only to the PEDV M protein in transfected cells. PEDV M protein expressed in two forms, one in stacking gel (marked as 2 in Figure) and the other as approximately 28Â kDa (marked as 1 in Figure). B As a form of validation, cells were transfected with HSP70 and PEDV M protein DNA only, and the analysis was performed again. All immunoprecipitation data clearly confirmed that immunoprecipitated PEDV M (number 1) was located at 28Â kDa, and PEDV M (number 2) was located in stacking gels in the input data.Back to article page