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Figure 6 | Veterinary Research

Figure 6

From: Heat shock protein 70 could enhance porcine epidemic diarrhoea virus replication by interacting with membrane proteins

Figure 6

Interaction between the PEDV M protein and HSP70. Cells were transfected with PEDV structure proteins. After 48 h, the cells were harvested, and immunoprecipitation was performed. The transfected protein molecular weights were as follows: PEDV E (13 kDa), PEDV M (28 kDa), PEDV N (55 kDa) and PEDV S (150 kDa). A Immunoprecipitation assays demonstrated that endogenous HSP70 bound only to the PEDV M protein in transfected cells. PEDV M protein expressed in two forms, one in stacking gel (marked as 2 in Figure) and the other as approximately 28 kDa (marked as 1 in Figure). B As a form of validation, cells were transfected with HSP70 and PEDV M protein DNA only, and the analysis was performed again. All immunoprecipitation data clearly confirmed that immunoprecipitated PEDV M (number 1) was located at 28 kDa, and PEDV M (number 2) was located in stacking gels in the input data.

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