Skip to main content
Figure 5 | Veterinary Research

Figure 5

From: Heat shock protein 70 could enhance porcine epidemic diarrhoea virus replication by interacting with membrane proteins

Figure 5

Comparison of HSP70 and PEDV protein expression in HSP70 knockdown and overexpressed cells. Vero cells were transfected with no siRNA (negative), scramble siRNA (scramble), or different siRNAs targeting HSP70 (siRNA576 and siRNA1070). Twenty-four hours later, the cells were mock-infected or infected with PEDV at an MOI of 1. A Western blot analysis of noninfected and PEDV-infected cells. B The viral titers of cell supernatants were determined 24 hpi. Data are mean ± SD (n = 3). C Vero cells were infected with a lentivirus control (NC) or pLenti-HSP70 (HSP70). Cells were harvested at 24 hpi for Western blot analysis. DE The levels of HSP70 and PEDV protein were quantified by determining band intensities and subsequent normalization to the levels of β-actin. Data are the mean ± SD (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).

Back to article page