Figure 3

Quercetin and heat shock treatment. A–D Vero cells were heated (HS) or not (Ctrl) or treated with quercetin (100 μM; QCT) or not (DMSO). Twelve hours later, cells were inoculated with PEDV at an MOI of 1. Cells were harvested at 12 h and 24 h for Western blot analysis. The levels of PEDV N protein were quantified by determining band intensities, which were then normalized to the levels of β-actin. Data are the mean ± SD (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001) A Cell lysates collected at 12 hpi were subjected to Western blot assay with anti-HSP70, anti-PEDV, and anti-β-actin antibodies. B Cell lysates collected at 24 hpi were subjected to Western blot assay with anti-HSP70, anti-PEDV, and anti-β-actin antibodies. C–D Quantification of 12 h and 24 h results.