Colocalization of HSP70 and PEDV. Vero cells were infected with PEDV at an MOI of 1. Cells were fixed at 24 hpi, and double immunofluorescence analysis was performed with anti-PEDV (green) and anti-HSP70 (red) antibodies. Mock treatments served as negative controls. The nuclei were stained with Hoechst 333258 (blue). A Anti- HSP70 staining of mock-infected cells. B Anti-PEDV staining of mock-infected cells. C Merged image. D Anti- HSP70 staining of PEDV-infected cells. E Anti-PEDV staining of PEDV-infected cells. F Merged image.