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Table 2 Full names and abbreviations of gene names, primer sequences, length of amplicons, annealing temperature and access to GenBank for selected reference genes

From: Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection

Reference genes

Gene

Forward primer

Reverse primer

Amplicon (bp)

Annealing temperature in real-time PCR [°C]

GenBank Accession Number

GAPDH (glyceraldehyde-3-phosphate dehydrogenase)

ACCACTTTGGCATCGTGGAG

GGGCCATCCACAGTCTTCTG

75

60°

U85042

HPRT (hypoxanthine–guanine phosphoribosyltransferase)

TGCTGAGGATTTGGAGAAGG

CAACAGGTCGGCAAAGAACT

154

60°

NW_001501830

  1. *Primers designed using Primer 3 0.4.0.