Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection

Table 1 Full names and abbreviations of gene names, primer sequences, length of amplicons, annealing temperature and access to GenBank, and/or source of chosen primers from the literature of the tested genes.

Gene	Forward primer (F) Reverse primer (R)	Amplicon (bp)	Annealing temperature [°C]	GenBank Accession Number	References
CXCL5	(F) TGAGACTGCTATCCAGCCG	193	61	AF149249	[34]
CXCL5	(R) AGATCACTGACCGTTTTGGG	193	61	AF149249	[34]
TNFα	(F) CGGTGGTGGGACTCGTATG	352	60	NM_173966.3	[35]
TNFα	(R) CTGGTTGTCTTCCAGCTTCACA	352	60	NM_173966.3	[35]
IL-18	(F) GAAAATGATGAAGACCTGGAATCA	84	60	NM_174091.2	[35]
IL-18	(R) AACTTGGTCATTCAATTTCGTATGA	84	60	NM_174091.2	[35]
IL-1β*	(F) AGAAAAGCCCGTCTTCCTGG	87	60	X12498.1	–
IL-1β*	(R) GGCTTTCTTTAGGGAGAGAGGG	87	60	X12498.1	–
IL-6*	(F) TGCAGTCTTCAAACGAGTGG	156	60	NM_173923.2	–
IL-6*	(R) TCTGACCAGAGGAGGGAATG	156	60	NM_173923.2	–
CCR1	(F) CTGCTGGTGATGATTGTCTG	191	61°	NM_001077839	[36]
CCR1	(R) TGCTCTGCTCACACTTACGG	191	61°	NM_001077839	[36]
CCL2	(F) CCCTCCTGTGCCTGCTACT	284	61°	NM_174006	[37]
CCL2	(R) TGCTCTGCTCACACTTACGG	284	61°	NM_174006	[37]

ISSN: 1297-9716