Figure 4

Laser confocal analysis of autophagy flux assessed by Ad-mCherry-GFP-LC3. A Representative images of mCherry-GFP-LC3 in bMEC. bMEC transfected by Ad-mCherry-GFP-LC3 were infected by M. bovis. The autophagy flux was analyzed by a laser confocal method. The mRFP⁺ GFP⁻ LC3 puncta yielded a red signal, whereas the mRFP⁺ GFP⁺ LC3 yielded a yellow signal. B Quantification of autophagosomes in bMEC. Twenty cells for each sample and at least 60 cells in each group were used for statistical analyses. SD of 3 independent experiments. Standard deviations of individual measurements are indicated as bars. ^*Compared to the control group (p  <  0.05).