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Figure 1 | Veterinary Research

Figure 1

From: Mycoplasma bovis subverts autophagy to promote intracellular replication in bovine mammary epithelial cells cultured in vitro

Figure 1

Cell infection model constructed. A Schematic overview. GM: gentamicin; stage I: bMEC infected (Time  =  0 h) with M. bovis inoculum at MOI of 1:30 for 1 h; stage II: extracellular M. bovis were killed by addition of 2 mL/well DMEM with 400 μg/mL gentamicin for 2 h at 37 °C; stage III: fresh DMEM with 10% fetal bovine serum (FBS) and 20 μg/mL gentamicin were added to the infected cells. MOI: multiplicity of infection. B Replication of intracellular M. bovis in the cell infection model. C Ultra-microstructure observation of adherent M. bovis. D Ultra-microstructure observation of intracellular M. bovis. Arrows point to M. bovis. Data are mean  ±  SD of 3 independent experiments. Standard deviations of individual measurements are indicated as bars. *Compared to the control group (p  <  0.05).

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