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Table 2 Oligonucleotide primers used in this study

From: LsrR, the effector of AI-2 quorum sensing, is vital for the H2O2 stress response in mammary pathogenic Escherichia coli

Primer name

Oligonucleotide (5′–3′)

DCM5-lsrR-f

ATCGTCTCGGCCTGACACGTTTGAAAGTGTCGCGATTGCTTGTAGGCTGGAGCTGCTT

DCM5-lsrR-r

TAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCTGAATATCCTCCTTAGTTC

DCM5-lacZ-f

ATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTTGTAGGCTGGAGCTGCTT

DCM5-lacZ-r

GACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTCATATGAATATCCTCCTTA

Check-lsrR-f

GTTGTTGCCCTCAATCTCC

Check-lsrR-r

ACTCCGCCTGTCCCACT

check-lacZ-f

GGCGGTGATTTTGGCGATAC

check-lacZ-r

GTAACGTTGGGTGCAAT

CM-f

TGTAGGCTGGAGCTGCTT

CM-r

CATATGAATATCCTCCTTAGTTC

pkatG-KpnI -f

GGGGTACCGTGAAAATCACACAGTGATC

pkatG-BamHI-r

CGGGATCCCAATGTGCTCCCCTCTACAG

pahpCF-HindIII-f

CCAAGCTTTAGATCAGGTGATTGCCCTT

pahpCF-BamHI-r

CGGGATCCCTATACTTCCTCCGTGTT

poxyR-HindIII-f

CCAAGCTTTCCGCAAAAGTTCACGTTGG

poxyR-BamHI-r

CGGGATCCTATCCATCCTCCATCGCCAC

pkatE-HindIII-f

CCAAGCTTTACTGGCTTCACTAAACGCA

pkatE-BamHI-r

CGGGATCCTGAACTCGTCTCCTTAATTT

pyciF-HindIII-f

CCAAGCTTACCGGAACCAGTTCAACACG

PyciF-BamHI-r

CGGGATCCAAGGTGGCTCCTACCCGTGA

prpoS-HindIII-f

CCAAGCTTCCTGATTCACCGTTAATTAT

prpoS-BamHI-r

CGGGATCCTTTTGCCAGTGCCCGGGTT

M6-pkatG-f

TTAACCGCGCGCATGTAAGATCTCAACTATCGCATCCG

M6-pkatG-r

TTACATGCGCGCGGTTAAAGAGATGTAGATCAAATTGATCT

M6-pahpCF-f

TTGTAAGGTGCGCGCTATCGATTTGATAATGGAAACGCA

M6-pahpCF-r

ATAGCGCGCACCTTACAACCTTCGTAAGACAACTTT

rt-16 s-f

TTTGAGTTCCCGGCC

rt-16 s-r

CGGCCGCAAGGTTAA

rt-lsrR-f

CGGATCGCGTGGTTTT

rt-lsrR-r

TCAACATATGCGCCGC

rt-ahpCF-f

AGGCATTCAAAAACGGCGAA

rt-ahpCF-r

CGTAGTGGTCAGCAACGTCA

rt-katG-f

GGTGTTGAGAAAGCCGCAAG

rt-katG-r

AGACGAGCGCGATAGTTACG

M13-f

TGTAAAACGACGGCCAGT

M13-r

CAGGAAACAGCTATGACC

T7-f

TAATACGACTCACTATAGGG

T7-r

TGCTAGTTATTGCTCAGCGG

lsrR-EcoRI-f

CCGGAATTCCGTTCAGTTTTGCAGGTGAG

lsrR-KpnI-r

CGGGGTACCTTAACTACGTAAAATCGCCG

lsrR-KpnI-f

GGGGTACCATGACAATCAACGATTCGGT

lsrR-EcoRI-r

CGGAATTCTTAACTACGTAAAATCGCCG

lsrR-NcoI-f

CATGCCATGGACAATCAACGATTCGGT

lsrR-XhoI-r

CCGCTCGAGACTACGTAAAATCGCCG

p-lsrR-biotin-f

ATTTCCCCCGTTCAGTTTTG

p-lsrR-r

AATTCATTCTTCACTTTGAA

p-oxyR-biotin-f

TCCGCAAAAGTTCACGTTGG

p-oxyR-r

TATCCATCCTCCATCGCCAC

p-ahpCF-biotin-f

TAGATCAGGTGATTGCCCTT

p-ahpCF-r

CTATACTTCCTCCGTGTT

p-katG-biotin-f

GTGAAAATCACACAGTGATC

p-katG-r

CAATGTGCTCCCCTCTACAG

  1. aThe underlined sequences refer to the restriction endonuclease recognition sites.