Figure 6

The binding ability of LsrR to the p-ahpCF-box, p-katG-box, and p-oxyR-box was determined by gel shift assays. In each panel, from lanes (1) to (5), the LsrR concentration was 8, 0, 2, 4, and 8 mmol, respectively; the amounts of biotin-labeled probes in all lanes were 100 fmol. In lane 1, besides the labeled probes, 1 pmol unlabeled probes were added as the competitive control (Ctrl). A Site1 and site2 represent two known binding boxes of LsrR. The red boxes indicate two putative binding sites of LsrR in the promoter regions of ahpCF and katG, respectively. B The binding ability of LsrR to its promoter (positive control). C The binding ability of LsrR to the ahpCF promoter. D The binding ability of LsrR to the katG promoter. E The binding ability of LsrR to the oxyR promoter.