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Figure 1 | Veterinary Research

Figure 1

From: LsrR, the effector of AI-2 quorum sensing, is vital for the H2O2 stress response in mammary pathogenic Escherichia coli

Figure 1

Molecular determination and growth curve assays of WT, lsrR mutant, and complementary strains. A Schematic diagram of the strategy for constructing the lsrR deletion mutant. B Confirmation of the wild-type strain WT/pSTV28, mutant strain XW10/pSTV28 and the complementary strain XW10/pClsrR. M: 2000 bp DNA marker; Lane 1: a 1660-bp PCR product was amplified from the strain WT/pSTV28 with primers check-lsrR-f/check-lsrR-r; Lane 2: a 1330-bp PCR product was amplified from the mutant strain XW10/pSTV28 with primers check-lsrR-f/check-lsrR-r; Lane 3: a 1330-bp PCR product was amplified from the complementary strain XW10/pClsrR with primers check-lsrR-f/check-lsrR-r; Lane 4: a 150-bp PCR product was amplified from the strain WT/pSTV28 with primers M13-f/M13-r; Lane 5: a 150-bp PCR product was amplified from the mutant strain XW10/pSTV28 with primers M13-f/M13-r; Lane 6: a 1094-bp PCR product was amplified from the complementary strain XW10/pClsrR with primers M13-f/M13-r. C Growth curves of strains WT/pSTV28, XW10/pSTV28, and XW10/pClsrR in LB broth with 15 μg/mL chloramphenicol. D Growth curves of strains WT/pUC19 and WT/pUClsrR in LB broth with 100 μg/mL ampicillin. The data represent the means of three independent assays.

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