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Figure 6 | Veterinary Research

Figure 6

From: On-farm colorimetric detection of Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in crude bovine nasal samples

Figure 6

Colorimetric results of in-lab loop-mediated isothermal amplification (LAMP) detection of bacteria in unprocessed mucus collected from steers. LAMP reactions were run in PCR tubes submerged in water at 65 °C inside a precision cooker. kmt1 detects PM, rsmL detects MH and lolB detects HS. The reactions were run for 60 min. DNA-free water was used as a negative control. Reactions were diluted in water. Images were collected with the Epson Perfection V800 Photo scanner and adjusted using the brightness/contrast tool on ImageJ and the white balance tool on Adobe Lightroom. See Additional file 13 for associated PCR results. PM, Pasteurella multocida; MH, Mannheimia haemolytica; HS, Histophilus somni.

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Veterinary Research

ISSN: 1297-9716