Table 1 Bacterial strains, plasmids, and primers used in this study.
Strain/plasmid/primer | Description |
|---|---|
Bacteria | |
JOL909 | S. Typhimurium Δlon (Lab stock) |
JOL2030 | S. Typhimurium Δlon ΔsifA |
E. coli DH5α | F-Φ80lacZM15 (lacZYA-argF) U169 recA1 endA1 hsdR17(rK)−, mK+ phoA supE44 thi-1 gyrA96 relA1 λ – (Promega) |
Plasmids | |
pKD3 | Template for frt-Cat-frt gene cassette |
pKD46 | Encodes recombinase enzyme |
pCP20 | Encodes flippase enzyme |
LentiCRISPR V2 | Provides CRISPR/Cas9 gene cassette |
CRISPR::pp38 | CRISPR/Cas9 system targeting pp38 gene |
CRISPR::meq | CRISPR/Cas9 system targeting meq gene |
Primers | |
FP sifA (sifA deletion) | TTGGTAAGGATTTCACTTTTTAAAAAACCATTCCCTATAGTAATCGGCAT GTGTAGGCTGGAGCTGCTTC |
RP sifA (sifA deletion) | AGTACGTGAGTAAACCCTGAACGTGACGTCTGAGAAAGCGTCGTCTGATT ATGGGAATTAGCCATGGTCC |