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Figure 5 | Veterinary Research

Figure 5

From: Genetic interference exerted by Salmonella-delivered CRISPR/Cas9 significantly reduces the pathological burden caused by Marek’s disease virus in chickens

Figure 5

Body weight, survival, and viral copy number of chickens after MDV challenge. A The body weight of chickens (n = 6) in all five groups was measured once a week for 9 weeks. Compared to the other groups, the ST-1st group demonstrated significant resistance against MDV infection and showed the smallest weight loss. *** indicates p < 0.05. B The survival rate of chickens in each test group. The chickens belonging to the ST-1st treatment group exhibited extended survival compared with those in the other groups upon MDV infection. C The qRT–PCR-measured changes in the threshold cycle (Ct) values of the meq and ovo genes in the chicken groups undergoing experimental MDV infection. Total RNA was extracted from PBMCs collected on the 7th, 14th, 21st, or 28th day post MDV infection for each chicken group, and cDNA was synthesized. The circulating MDV viral load was determined by a qRT–PCR-based assay using the meq gene as the reference gene. An increasing MDV viral load was demonstrated by reduced Ct values in all samples with time. The viral loads in the ST-1st group showed significant differences beyond Day 21 post-infection. The change in the Ct values of the internal housekeeping ovo gene is shown. *** indicates a significant difference compared to the PBS control group. P < 0.05. The average CT values and standard deviation of three independent trials are shown.

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