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Table 2 Primer sequences for RT-qPCR.

From: MiR-125b regulates inflammation in bovine mammary epithelial cells by targeting the NKIRAS2 gene

Gene symbol

Primer sequence

Amplicon (bp)

References

NKIRAS2

F: 5′-GGAGCCCTTCGTCTACCT-3′

R: 5′-GCACCACTTCAGCCATCC-3′

111

NF-κB

F: 5′-AAGAGCCCTTTCAATGGACC-3′

R: 5′-GTGCTGAGAGATGGCGTAAA-3′

128

IL-6

F: 5′-CTGGGTTCAATCAGGCGAT-3′

R: 5′-CAGCAGGTCAGTGTTTGTGG-3'

205

[19]

TNF-α

F: 5′-TCTGGGCAGGTCTACTTTG-3′

R: 5′-CCTGAGCCCATAATTCCCT-3′

139

UXT

F: 5′-CATTGAGCGACTCCAGGAAG-3′

R: 5′-GGCCACATAGATCCGTGAAG-3′

112

[22]

GADPH

F: 5′-GGCATCGTGGAGGGACTTATG-3′

R: 5′-GCCAGTGAGCTTCCCGTTGAG-3′

186

[22]

miR-125b

RT: 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACTCACAAGT-3′

F: 5′-GCATCCTCCCTGAGACCCTA-3′

R: 5′-CAGTGCAGGGTCCGAGGTAT-3′

64

  1. F (Forward) primer and R (Reverse) primer were used for qPCR assays. RT primer is used for reverse transcription of miR-125b.