Figure 4
From: MiR-125b regulates inflammation in bovine mammary epithelial cells by targeting the NKIRAS2 gene
Bovine miR-125b suppresses NKIRAS2 expression in MAC-T cells. A Transfection efficiency of miR-125b mimic or inhibitor in MAC-T cells as observed by fluorescence microscopy. B The transfection efficiency of bovine miR-125b mimic or inhibitor in MAC-T cells as measured by qPCR. C qPCR analysis of NKIRAS2 in MAC-T cells transfected with miR-125b mimic or inhibitor. D Western blot analysis of NKIRAS2 in MAC-T cells transfected with miR-125b mimic or inhibitor. Western blot images were analyzed using the Image J software, and densitometry quantification of western blots were normalized to β-actin. Data are presented as mean ± SD. **: adjusted p value < 0.01, ***: adjusted p value < 0.001, ****: adjusted p value < 0.0001, as determined by as determined as determined by one-way ANOVA multiple comparisons. mimic = miR-125b mimic, Inhibitor = miR-125b inhibitor, NC = negative control, compared with miR-125b mimic and inhibitor.