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Figure 2 | Veterinary Research

Figure 2

From: MiR-125b regulates inflammation in bovine mammary epithelial cells by targeting the NKIRAS2 gene

Figure 2

Bovine miR-125b directly targets NF-κB inhibitor interacting RAS-like 2 (NKIRAS2) gene by binding to its 3′ untranslated region (3′ UTR). A The predicted miR-125b binding sites within 3′ UTR of NKIRAS2 and the experimentally introduced mutations are shown. B Human embryonic kidney (HEK) 293 cells were transiently co-transfected with CHECK2-3′UTR dual-luciferase reporter recombinant vector, and bovine miR-125b mimic or NC_mimic. The data in legend are the relative luciferase activity (Renilla / Firefly), in which, renilla luciferase activity was normalized to the activity of firefly luciferase in the same recombination vector. Data are Mean ± SD. **: adjusted p value < 0.01; ns: adjusted p value > 0.05. NC_mimic = negative control, compared with miR-125b mimics; CHECK2_3′UTR = NKIRAS2-3UTR-wt (or -mut) and psiCHECK™-2 dual luciferase reporter recombinant vector.

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