Table 2 Primers for PCR detection of E. coli O157:H7 in rat faeces
Primer | Primer sequence (5′–3′) | Target gene | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|
O157-F | AAGATTGCGCTGAAGCCTTTG | rfbE | 55 | 497 |
O157-R | CATTGGCATCGTGTGGACAG | rfbE | 55 | 497 |
H7-F | GCGCTGTCGAGTTCTATCGAGC | fliC | 55 | 625 |
H7-R | CAACTGTGACTTTATCGCCATTCC | fliC | 55 | 625 |