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Table 1 Parameters of the orthogonal tests for phage microencapsulation

From: Microencapsulated phages show prolonged stability in gastrointestinal environments and high therapeutic efficiency to treat Escherichia coli O157:H7 infection

No.

A (%)

B

C (mm)

D (%)

E (rpm)

EE (%)

1

3

1:2

1.2

3

0

26.710  ±  1.191

2

2

1:1

0.5

3

500

32.280  ±  0.481

3

1

1:2

0.9

2

500

29.700  ±  1.018

4

1.5

2:1

0.5

2

200

41.057  ±  1.592

5

2.5

4:1

0.7

3

200

30.753  ±  1.005

6

1

4:1

0.5

1

0

55.423  ±  4.356

7

2

4:1

0.9

4

300

30.660  ±  0.407

8

1.5

1:1

0.6

4

0

34.180  ±  1.374

9

1.5

1:2

0.7

1

300

48.633  ±  1.653

10a

3

4:1

0.6

2

100

77.767  ±  2.636

11

2.5

2:1

0.9

5

0

27.995  ±  1.270

12

3

1:1

0.9

1

200

34.028  ±  1.867

13

1.5

1:4

0.9

3

100

24.120  ±  1.559

14

2.5

1:1

1.2

2

300

25.707  ±  1.494

15

1.5

4:1

1.2

5

500

38.097  ±  1.962

16

2

2:1

1.2

1

100

55.140  ±  1.702

17

2

1:2

0.6

5

200

30.917  ±  0.820

18

3

1:4

0.5

5

300

26.576  ±  1.134

19

2.5

1:2

0.5

4

100

29.537  ±  1.788

20

2.5

1:4

0.6

1

500

29.930  ±  0.853

21

3

2:1

0.7

4

500

59.243  ±  2.671

22

1

1:1

0.7

5

100

25.440  ±  0.421

23

1

2:1

0.6

3

300

44.530  ±  2.071

24

1

1:4

1.2

4

200

27.153  ±  1.940

25

2

1:4

0.7

2

0

29.290  ±  1.095

  1. A: concentration of sodium alginate; B: ratio of phage to sodium alginate; C: syringe needle diameter; D: concentration of CaCl2; E: speed of stirring; EE: encapsulation efficiency (mean ± SD, n = 3).
  2. aPreparation parameters with the highest encapsulation efficiency.