High-frequency and activation of CD4+CD25+ T cells maintain persistent immunotolerance induced by congenital ALV-J infection

Table 2 Primers for qRT-PCR amplification

Purpose gene	Primer direction	Primer sequences (5′-3′)	Product length (bp)
IL-2	Forward	TTCATCTCGAGCTCTACACACCAA	200
IL-2	Reverse	GCATTCACTTCCGGTGTGATTTA	200
IL-10	Forward	GAGCTGAGGGTGAAGTTTGAGGA	85
IL-10	Reverse	GTTCAGAGCTGAGCAGTTGGATGT	85
IFN-γ	Forward	AGCATTTGAACTGAGCCATCACC	181
IFN-γ	Reverse	CCGTCAGCTACATCTGAATGACTTG	181
TGF-β	Forward	ACCTCGACACCGACTACTGCTTC	126
TGF-β	Reverse	CCATATAACCTTTGGGTTCGTGGA	126
CTLA-4	Forward	TCTGCAAGATGGAGCGGATG	174
CTLA-4	Reverse	CGACAATGGCTGAGATGATGATG	174

ISSN: 1297-9716