From: High-frequency and activation of CD4+CD25+ T cells maintain persistent immunotolerance induced by congenital ALV-J infection
Purpose gene
Primer direction
Primer sequences (5′-3′)
Product length (bp)
env gene of ALV-J
Forward
TGCGTGCGTGGTTATTATTTC
144
Reverse
AATGGTGAGGTCGCTGACTGT
GAPDH gene of chicken
GAACATCATCCCAGCGTCCA
132
CGGCAGGTCAGGTCAACAAC