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Table 1 Primers for routine PCR amplification

From: High-frequency and activation of CD4+CD25+ T cells maintain persistent immunotolerance induced by congenital ALV-J infection

Purpose gene

Primer direction

Primer sequences (5′-3′)

Product length (bp)

env gene of ALV-J

Forward

TGCGTGCGTGGTTATTATTTC

144

Reverse

AATGGTGAGGTCGCTGACTGT

GAPDH gene of chicken

Forward

GAACATCATCCCAGCGTCCA

132

Reverse

CGGCAGGTCAGGTCAACAAC