Figure 5

CD4⁺CD25⁺ Tregs disrupts the proliferation and differentiation of B cells after ALV-J infection. A Histological structure of the chicken spleen. B FACS analyses of the proportion of B cells in the spleen of chickens. C Quantitation of B. D, E CLSM detection of CD4⁺CD25⁺ Tregs in the spleen of chickens. I IHC detection of B cells (Bu-1⁺ cells, black purple) and PNA⁺ cells (GC, brown). G, H Quantitation of F. I IHC detection of IgM⁺ plasma cells (brown) or and IgG⁺ plasma cells (red). J Quantitation of I **p < 0.01, ***p < 0.001, two-way ANOVA was performed, data were expressed as the mean ± SEM.