Figure 4From: High-frequency and activation of CD4+CD25+ T cells maintain persistent immunotolerance induced by congenital ALV-J infectionCongenital ALV-J infection causes an increase in the frequency of CD4+CD25+Tregs and high viral load. A FACS analysis strategy for CD4+CD25+ Tregs. B Quantitation of the frequency of CD4+CD25+ Tregs in blood and immune organs. C CLSM detection of CD4+CD25+ Tregs in the thymus of chicken. D ELISA results of viral load of ALV-J in immune organs. *p < 0.05, **p < 0.01, ***p < 0.001, the unpaired t-test was performed, and the data were expressed as the mean ± SEM.Back to article page