Figure 2

Growth analysis of E. tarda in different conditions. The wild-type TX01, its isogenic cadA, cadB and cadBA deletion mutant strains TX01ΔcadA, TX01ΔcadB, and TX01ΔcadBA were cultured to the exponential phase. After diluting serially, bacteria were cultured in fresh LB broth with pH adjusted to 7.0, 5.5, 5.0, and 4.5 (A–D). Bacteria were added into fresh medium with 500 μM of diamide (E) and 50 μM of 2,2ʹ-dipyridyl (Dp) (F), respectively. Cell density was monitored at 2-h intervals by measuring the OD₆₀₀. Experiment was performed three times, data are presented as the means ± SEM (N = 3). N, the number of times the experiment was performed.