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Figure 1 | Veterinary Research

Figure 1

From: Acid resistance system CadBA is implicated in acid tolerance and biofilm formation and is identified as a new virulence factor of Edwardsiella tarda

Figure 1

Schematic organization and co-transcriptional verification of the cadBA operon in E. tarda. A Schematic organization of the cadBA operon in E. tarda. The cadBA operon consists of cadB and cadA. The cadBA operon is under the control of the predictive promoter Pcad. The upstream gene of the cadBA operon is cadC, which encodes transcriptional regulator. Therefore, the operon was named cadBA. B Verification of cadB and cadA co-transcription. Genomic DNA and total RNA were isolated from overnight cultures of E. tarda. RNA was treated with DNase I and cDNA was synthesized. PCR were conducted with specific primer pair CadBAF/CadBAR using genomic DNA, cDNA, and RNA as the template. PCR products were analyzed by agarose gel electrophoresis.

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