Table 1 Oligonucleotide primers used for the detection of genes related to biofilm formation (bap, ica, and aap) and quorum sensing (agr)
Gene | Nucleotide sequence | Amplification conditions | References |
|---|---|---|---|
bap | 5′-ACTTAYTRCCHTATATCGAARTAG-3′ | 94 °C 30 s, | [27] |
5′-GCTGTTGAAGTTAATACTGTACCTGC-3′ | 57 °C 30 s, | ||
72 °C 30 s, | |||
30 cycles | |||
ica | 5′-CTGTTTCATGGAAACTCC-3′ | 94 °C 30 s, | [44] |
5′-TCGATGCGATTTGTTCAAACAT-3′ | 57 °C 30 s, | ||
|  | 72 °C 30 s, | ||
30 cycles | |||
aap | 5′-GAAGCACCGAATGTTCCAACTATC-3′ | 94 °C 30 s, | [28] |
5′-AGTTGGCGGTATATCTATTGTA-3′ | 54 °C 30 s, | ||
72 °C 30 s, | |||
30 cycles | |||
agr | 5′-CATAGCACTGAGTCCAAGGA-3′ | 94 °C 30 s, | [45] |
5′-CAATCGGTGACTTAGTAAAATG-3′ | 55 °C 30 s, | ||
72 °C 60 s, | |||
30 cycles |