Specific binding of rTsGSCP with IECs and cellular localization. A IIF analysis showing the specific binding of rTsGSCP with IECs. IECs or C2C12 cells were preincubated with rTsGSCP or MBP. After blocking and washing, the cells were probed with anti-rTsGSCP serum, infection serum, anti-MBP-tag serum or preimmune serum, followed by incubation with FITC-conjugated anti-mouse IgG. Cell nuclei were stained blue with DAPI and examined by fluorescence microscopy (400 ×). Scale bars, 25 μm. B Cellular localization of rTsGSCP within IECs as shown at high magnification (1000 ×). The IECs were preincubated with rTsGSCP, subsequently incubated with anti-rTsGSCP serum, infection serum or preimmune serum, and finally stained with FITC-conjugated anti-mouse IgG. DAPI was applied to stain cell nuclei blue. Scale bars, 5 μm.