Far-Western blot identification of binding between rTsGSCP and IEC proteins. A SDS-PAGE analysis of soluble IEC proteins (lane 1) and C2C12 cell proteins (lane 2). Lane M, protein marker. B Far-Western blotting showing the binding of rTsGSCP with IEC proteins. Lane M, protein marker. The strips containing IEC protein (lanes 1–6) were incubated with rTsGSCP (lanes 1–3) or MBP (lanes 4–6). Bands of indicative of rTsGSCP binding with IECs were identified with anti-rTsGSCP serum (lane 1) and infection serum (lane 2) but not with preimmune serum (lane 3). No binding of MBP with IECs was detected with anti-MBP serum (lane 4), infection serum (lane 5) or preimmune serum (lane 6). There was no binding of rTsGSCP with C2C12 cells when anti-rTsGSCP serum (lane 7), infection serum (lane 8) or preimmune serum (lane 9) was used.