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Figure 3 | Veterinary Research

Figure 3

From: Characterization of a novel cysteine protease in Trichinella spiralis and its role in larval intrusion, development and fecundity

Figure 3

Expression and antigenicity analyses of rTsGSCP. A SDS-PAGE gel showing rTsGSCP. Lane M, protein marker; lane 1, lysate of bacteria carrying pMAL-c2x/TsGSCP prior to induction; lane 2, lysate of induced bacteria carrying pMAL-c2x/TsGSCP; lane 3, purified pMAL-c2x/TsGSCP. B SDS-PAGE gel showing of worm antigens. Lane M, protein marker; lane 1, IIL soluble crude proteins; lane 2, IIL ES proteins; lane 3, purified rTsGSCP. C Western blot analysis of rTsGSCP. IIL soluble protein (lane 1), IIL ES protein (lane 2) and rTsGSCP (lane 3) were recognized with infection serum. Natural TsGSCP in IIL crude proteins (lane 4) and rTsGSCP (lane 6) were identified with anti-rTsGSCP serum, but native TsGSCP was not found among IIL ES proteins (lane 5) when probed with anti-rTsGSCP serum. Crude IIL (lanes 7 and 10), ES proteins (lanes 8 and 11), and rTsGSCP (lanes 9 and 12) were not recognized by preimmune serum (lanes 7–9) or anti-MBP-tag serum (lanes 10–12).

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Veterinary Research

ISSN: 1297-9716