Suppression of TsGSCP expression and enzymatic activity after dsRNA interference. A Various doses of dsRNA-TsGSCP inhibited TsGSCP transcription. B Various doses of dsRNA-TsGSCP inhibited TsGSCP expression. C TsGSCP transcription level on different days after dsRNA transfection. D TsGSCP expression level as measured on different days after dsRNA transfection. E Expression levels of TsGSCP and T. spiralis aspartyl aminopeptidase (TsAAP) in ML treated using TsGSCP-specific dsRNA. F The ability of TsGSCP to enzymatically cleave substrate Z-FR-AMC was significantly reduced in dsRNA-TsGSCP-treated ML and IIL. *P < 0.05 relative to the PBS group.