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Table 1 Primers used for gene cloning and expression analysis

From: Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells

Primer namea

Primer sequence (5’-3’)

chTERT-T1-F (Xba I)

CTCTACTAGAGGATCtctagaCGTGGGGCCCGCTGCACGGCAGCGb

chTERT-T1-R

ATACGCAGTCATTCACTCTCATCTTCCACATC

chTERT-T2-F

GCCATAACAAATGCCGGTTCTTTAAAAACGTG

chTERT-T2-R

GGGGTACCAGACCTTCATCCCTTAGTCCAG

chTERT-T2-linker-F

GTGATCCGCTCCTCAGGGAGCTGCTCAGGCAGCACAGCA

GCCACTGGCAGGTGTATGGCTTTGTGAGGG

chTERT-T2-linker-R

CGATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCC

TCCACCGGTACCGTCCAGTATAGTTTTGAAAT

chTERT-T2-HA-F

GTGATCCGCTCCTCAGGGAGCTGCTCAGGCAGCACAGCA

GCCACTGGCAGGTGTATGGCTTTGTGAGGG

chTERT-T2-HA-R

(Xho I)

GGGCCCGGGTTCGAActcgagTTAAGCGTAGTCTGGGACGT

CGTATGGGTATCGCGACGATCCGCCACCGCCAGAGCCAC2

  1. aF: forward primer, R: reverse primer
  2. bThe lowercase letters indicate restriction sites
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Veterinary Research

ISSN: 1297-9716