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Figure 9 | Veterinary Research

Figure 9

From: Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells

Figure 9

chTERT promoted the replication of ALV-J in LMH cells. A Seventy-two hours after LMH-chTERT and LMH-NC cells were infected with the same amount of ALV-J (CHN06), the level of ALV-J in the cell pellet was measured by RT-PCR and Western blotting. B LMH-chTERT cells were transfected with chTERT and β-catenin siRNAs and then inoculated with the same amount of ALV-J. After 72 h of incubation, the ALV-J level was measured by RT-PCR and Western blotting. The figure shows that after inhibiting chTERT gene expression or β-catenin signalling pathway activity, the replication of ALV-J was inhibited. C LMH-chTERT and LMH-NC cells were infected with the same amount of ALV-J virus. After 2 h of incubation, the virus solution was discarded and replaced with new culture medium containing 1% FBS for 7 days. Then, the supernatant was collected every day to detect the p27 antigen. D The effect of chTERT on the virus titre of ALV-J. E LMH-chTERT cells were infected with ALV-J at an MOI of 0.01, 0.1 or 1.0. After 72 h of incubation, the expression of chTERT in the cell pellets of the infected and uninfected groups was measured by RT-PCR and Western blotting, and telomerase activity was also assessed (F).

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