chTERT inhibited the apoptosis of LMH cells. A, B The mRNA levels of genes that promote apoptosis (Caspase 3, Caspase 9, and BAX) or inhibit apoptosis (BCL-2 and BCL-X) were measured by RT-PCR. C Flow cytometry analysis of early apoptotic, late apoptotic and necrotic LMH cells. APC-positive and 7-AAD-negative cells represent early apoptotic cells, and 7-AAD-positive cells represent late apoptotic or necrotic cells. D TUNEL staining was used to analyse late apoptotic LMH cells. In late apoptotic cells, chromosomal DNA breaks result in the production of a large number of sticky 3'-OH ends. Under the action of deoxyribonucleotide terminal transferase (TdT), deoxyribonucleotides, fluorescein, peroxide derivatives, alkaline phosphatase, or biotin are added to the 3'-end of the DNA, allowing apoptotic cells to be detected.