Figure 5

chTERT promoted the proliferation of LMH cells. A The cell cycle was analysed by flow cytometry. G1, the prophase of DNA synthesis. S phase, the DNA synthesis phase. G2, late phase of DNA synthesis. B Cell proliferation at 24, 48 and 72 h was analysed by flow cytometry. C The MTT assay was used to analyse the viability of LMH-chTERT and LMH-NC cells over 1–5 days. Growth curves were drawn after the values were read at a wavelength of 450 nm. D The MTT assay was used to analyse the viability of LMH-chTERT cells 72 h after transfection with siRNA. E Immunofluorescence staining of Ki67, an antigen associated with proliferating cells. Ki67 is closely related to mitosis and is indispensable for cell proliferation.