Table 2 Primers and TaqMan probe sequences used for RT-qPCR
Cell type | Genes | NCBI Reference | TaqMan/SYBR-Green | Type | Sequence (5ʹ-3ʹ) |
|---|---|---|---|---|---|
NK cells | NFIL3 | XM_017014743.1 | SYBR-Green | Forward | TGAATGCCATCAGTTGAGC |
Reverse | GAGAGGCGGAGAATGTGAGT | ||||
IL-7Rα | NM_001080106.1 | SYBR-Green | Forward | ATTCTGGGAAAGCAGGATCAAG | |
Reverse | CTTACACAGTCGCTCCAGAGTTATTT | ||||
PRF1 | XM_004945690.3 | SYBR-Green | Forward | ACCCGCACCAAAAGATGAAG | |
Reverse | TAATTCGCACACCCCTAAACG | ||||
GAPDH | NM_204305.1 | SYBR-Green | Forward | GTGGTGCTAAGCGTGTTATC | |
Reverse | GCATGGACAGTGGTCATAAG | ||||
APCs | CD14 | NM_001139478.1 | TaqMan | Forward | GGACGACTCCACCATTGACAT |
Reverse | GGAGGACCTCAGGAACCAGAA | ||||
Probe | AATGATCTTCCTGATTTGCAGACTGCCAA | ||||
TLR4 | NM_001030693.1 | SYBR-Green | Forward | GTCCCTGCTGGCAGGAT | |
Reverse | TGTCCTGTGCATCTGAAAGCT | ||||
MERTK | NM_204988.1 | SYBR-Green | Forward | TGTGGAAGGATGGCAGGGAG | |
Reverse | GCACGGATGCTGAATGTAGAGG | ||||
MAFB | NM_001030852.1 | SYBR-Green | Forward | AGGACCGGTTCTCGGATGAC | |
Reverse | CCTCGGAGGTGCCTGTTG | ||||
INOS | NM_204961.1 | SYBR-Green | Forward | TGGGTGGAAGCCGAAATA | |
Reverse | GTACCAGCCGTTGAAAGGAC | ||||
ZBTB46 | XM_015296613.2 | SYBR-Green | Forward | CTGGACCTGTGGAAGAGGAAAC | |
Reverse | CGGTAGTGGGAGGCAATCTC | ||||
XCR1 | NM_001024644.2 | SYBR-Green | Forward | CCTTCGGGTGGATTTTTGGT | |
Reverse | CGCTGTAGTAGCCAATGGAGAA | ||||
FLT3 | NM_004119.3 | SYBR-Green | Forward | CATTCGGACCCAGTACATGTTTAC | |
Reverse | TGAGCCGTAGAAGAGCAGGTATAA | ||||
GAPDH | NM_204305.1 | SYBR-Green | Forward | GTGGTGCTAAGCGTGTTATC | |
Reverse | GCATGGACAGTGGTCATAAG | ||||
28S | XR_00378040.1 | TaqMan | Forward | GGCGAAGCCAGAGGAAACT | |
Reverse | GACGACCGATTTGCACGTC | ||||
Probe | AGGACCGCTACGGACCTCCACCA |