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Table 1 Flow cytometry staining reagents

From: A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens

Cell population Primary antibody (mouse-anti-chicken) Clone/isotype Secondary antibody
NK cells CD45-FITC1 LT40/IgM
CD3-APC1 CT3/IgG1
IL-2Rα-UNL2 28-4/IgG3 Goat-anti-mouse-IgG3-PE1
20E5-BIOT2 IgG1 Streptavidin (SA)-PercP6
T cells CD3-PE1 CT3/IgG1
CD4-APC1 CT4/IgG1
TCRγδ-FITC1 TCR-1/IgG1
CD8α-UNL1 EP72/IgG2b Goat-anti-mouse-IgG2b-APC/Cy71
CD8β-BIOT1 EP42/IgG2a SA-PercP6
APCs CD41/61-FITC4 11C3/IgG1
Bu-1-AF6471 AV20/IgG1
CD3-FITC1 CT3/IgG1
CD4-PE/Cy71 CT4/IgG1
MRC1LB-PE1 KUL01/IgG1
CD11-biotin2 5C7/IgG1 SA-Brilliant Violet (BV) 6057
MHC-II-UNL1 Cia/IgM Rat-anti-mouse-IgM-BV421 (RMM-1)7
*CHIR-AB1-UNL2 8D12/IgG2a Rat-anti-mouse-IgG2a-PerCP/Cy5.5 (RMG2a-62)7
*CD40-UNL5 AV79/IgG2a Rat-anti-mouse-IgG2a-PerCP/Cy5.5 (RMG2a-62)7
*CD80-UNL5 IAH:F864:DC7/IgG2a Rat-anti-mouse-IgG2a-PerCP/Cy5.5 (RMG2a-62)7
Assay
 CD107 CD107a-APC3 LEP-100 I 5G10/IgG1
CD41/61-FITC4 11C3/IgG1
CD3-PE1 CT3/IgG1
CD8α-UNL1 EP72/IgG2b Goat-anti-mouse-IgG2b-Alexa Fluor (AF) 7908
28–4-UNL2 IgG3 Goat-anti-mouse-IgG3-APC/Cy71
20E5-BIOT2 IgG1 SA-PercP6
 IFNγ CD3-PE1 CT3/IgG1
TCRγδ-FITC1 TCR-1/IgG1
CD8α-UNL1 EP72/IgG2b Goat-anti-mouse-IgG2b-AF7908
28-4-UNL2 IgG3 Goat-anti-mouse-IgG3-APC/Cy71
20E5-BIOT2 IgG1 SA-PercP6
IFNγ-APC3 MAb80/IgG1
 T cell proliferation CD3-FITC1 CT3/IgG1
CD4-PE/Cy71 CT4/IgG1
CD8α-APC1 CT8/IgG1
IL-2Rα-UNL2 28-4/IgG3 Goat-anti-mouse-IgG3-PE1
  1. Three APC antibody panels were prepared each containing surface markers plus one out of three antibodies indicated (*).
  2. Manufacturer: 1Southern Biotech, AL, USA.
  3. 2Purified antibody from hybridoma supernatant [44], kindly provided by Göbel, T.W., Ludwig Maximilian University, Germany.
  4. 3Developmental Studies Hybridoma Bank (DSHB), University of Iowa, IA, USA.
  5. 4Serotec, United Kingdom.
  6. 5Bio-Rad.
  7. 6BD Biosciences.
  8. 7Biolegend.
  9. 8Jackson ImmunoResearch Laboratories, PA, USA.