Figure 5

Activation marker expression by splenic APC subsets upon SE infection. A Macrophages, B FSC^low DCs and C FSC^high DCs were assessed over time for CHIR-AB1, D-F CD40, G-I CD80 and J-L MHC-II expression in uninfected (uninf) and SE-infected (SE-inf) chickens. For CHIR-AB1, CD40 and CD80, the percentage of cells in each APC subset expressing the respective markers is shown, and for MHC-II the geometric mean fluorescent intensity (gMFI) of each subset, in accordance with the gating strategy depicted in Additional file 5. Mean + SEM per treatment and time point is shown (n = 5), for uninfected chickens at 0 dpi n = 3 and at 7 dpi n = 4. Statistical significance is indicated as * p < 0.05, ** p < 0.01, **** p < 0.0001.