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Figure 5 | Veterinary Research

Figure 5

From: Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics

Figure 5

Relative expression of IL-8, TGF-β and TLRs in equine enteroids and 2D monolayers. The gene expression of IL-8, TGF-β (A), TLR2, TLR4 and TLR5 (B) in the monolayer cultures was normalised to the geometric mean for the reference genes (HPRT, SDHA and GAPDH) and calibrated to that in the enteroids. The results are given as mean values ± SD for cultures established from each of the three experimental horses (n = 3). P < 0.05 = * and P < 0.01 = **. FC > 2 shown by dashed line indicates gene up-regulation. nd = not detected.

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