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Figure 4 | Veterinary Research

Figure 4

From: Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics

Figure 4

Cellular composition after transfer of equine enteroids to 2D monolayers. Enteroids could be dissociated to single cells and re-established on pre-coated plates or transwell inserts. A Graphical illustration of enteroid and monolayer cultures. B Monolayer after three days of culture on a flat-bottomed plate. Contrast stained by hematoxylin (Photo: Nikon D40x). C Transepithelial electrical resistance in transwell cultures monitored over 15 days (mean value ± SD, two technical replicates). D Expression of the cell lineage markers SOX9, LYZ, PCNA, EPCAM, CGA, MUC2 and DCLK1 in enteroids or in 2D monolayers cultured on plates or transwell inserts. The gene expression was normalized to the geometric mean for the reference genes (HPRT, SDHA and GAPDH) and is presented as 2−ΔCt. The results are given as mean values ± SD for cultures established from each of the three experimental horses (n = 3). P < 0.05 = * and P < 0.001 = ***.

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