Figure 1
Lipidomic profiling from prion-infected mice. A Overall workflow for PrPSc detection and lipidomic profiling from prion-infected mice. IP: intraperitoneal; LC/MS: liquid chromatography mass spectrometry B Western blotting detection of PrPSc in the brain. The prion protein (PrP) was detected with mouse monoclonal anti-PrP antibody SAF84 (1:200; Cayman, USA). Lane 1: Proteinase K-untreated whole brain from C57BL/6 mice inoculated with PBS (CTL) at 3 months post-injection. Lane 2: Proteinase K-treated whole brain from C57BL/6 mice inoculated with PBS at 3 months post-injection. Lane 3: Proteinase K-untreated whole brain from C57BL/6 mice inoculated with ME7 scrapie (ME7) at 3 months post-injection. Lane 4: Proteinase K-treated whole brain from C57BL/6 mice inoculated with ME7 scrapie at 3 months post-injection. Lane 5: Proteinase K-untreated whole brain from C57BL/6 mice inoculated with PBS at 7 months post-injection. Lane 6: Proteinase K-treated whole brain from C57BL/6 mice inoculated with PBS at 7 months post-injection. Lane 7: Proteinase K-untreated whole brain from C57BL/6 mice inoculated with ME7 scrapie at 7 months post-injection. Lane 8: Proteinase K-treated whole brain from C57BL/6 mice inoculated with ME7 scrapie at 7 months post-injection. Bars on the left indicate molecular size markers (kDa). -: proteinase K-untreated lanes; +: proteinase K-treated lanes.