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Table 3 Besnoitia spp. detection by means of PCR and histological techniques in testicle tissues of chronically infected bulls

From: Identification of molecular biomarkers associated with disease progression in the testis of bulls infected with Besnoitia besnoiti

Bull Pampiniform plexus Testicular parenchyma Scrotal skin
PCR qPCR (zoites/mg tissue) Histology (mean cysts diameter (µm) PCR qPCR (zoites/mg tissue) Histology (mean cysts diameter (µm) PCR qPCR (zoites/mg tissue) Histology (average cysts diameter; µm)
8 POS 4.5 × 10–5 272.71 POS 3.66 POS 2 × 10–5 232.74
9 POS 6.4 × 10–5 225.00 POS 51.12 225.00 POS 4017.92 253.17
10 POS nd 150.09 POS 9.7 × 10–1 POS nd 121.08
11 POS 1.2 × 10–4 196.76 POS 393.09 189.46 POS 13,442.67 316.91
12 POS 1.3 × 10–1 142.43 POS 8.08 POS 3818.31 183.90
13 POS 1.2 × 10–1 133.96 POS 2.4 × 10–1 POS 2130.38 210.69
14 POS 1 × 10–4 171.44 POS 1.09 168.43 POS 1.6 × 10–2 236.38
15 POS 9.5 × 10–2 126.41 POS 3.3 × 10–1 POS 7.7 × 10–3 252.69
16 POS 9.7 × 10–3 174.56 POS 4.2 × 10–1 POS 1899.65 235.59
17 POS 8.6 × 10–2 176.95 POS 11.45 POS 3017.55 327.69
  1. POS, detection of Besnoitia spp. DNA; nd, not determined; –,cysts not found.