|
Gene
|
Forward
|
Reverse
|
Accession
|
Amplicon size (bp)
|
|---|
|
IL1β
|
CAGTCTTCAGTGCTCAGGTTTCTG
|
CATTGCCGCTGCAGTAAGT
|
XM_001495926.4
|
84
|
|
IL1RN
|
GCCTGTGTCAAGTCTGGTGA
|
ACTCTTTGGGCTTGTTGGTG
|
XM_005599766.3
|
217
|
|
IL6
|
GGATGCTTCCAATCTGGGTTCAAT
|
TCCGAAAGACCAGTGATGATTTT
|
NM_001082496.2
|
65
|
|
PLAC8
|
GTTTCATGTGCCTTGCGTTGT
|
ATATCGGGTCCGGTAGAGGG
|
XM_003364673.4
|
98
|
|
SAA1
|
CCTGGGCTGCTAAAGTCATC
|
AGGCCATGAGGTCTGAAGTG
|
XM_023646155.1
|
169
|
|
INHBA
|
CCTCCTCTTCCTTCTTCTTCTTCTT
|
GCAAGAGCTCCCTGGATATT
|
XM_023638729.1
|
99
|
|
GUSB
|
GGGATTCGCACTGTGGCTGTCA
|
CCAGTCAAAGCCCTTCCCTCGGA
|
XM_001493514
|
117
|
|
ACTB
|
CGACATCCGTAAGGACCTGT
|
CAGGGCTGTGATCTCCTTCT
|
NM_001081838
|
100
|
- Primers were generated using Primer-BLAST (NCBI).
- Key: RT-qPCR, Real time quantitative polymerase chain reaction; NCBI, National Center for Biotechnology Information; IL1β, Interleukin 1β; IL1RN, Interleukin 1 Receptor Antagonist; IL6, Interleukin 6; PLAC8, Placenta-specific 8; SAA1, Serum Amyloid A1; INHBA, Inhibin Subunit Beta A; ACTB, actin beta; GUSB, Glucuronidase Beta.
