Transcriptomic analysis of equine chorioallantois reveals immune networks and molecular mechanisms involved in nocardioform placentitis

Table 1 Forward and reverse primer sequences used for RT-qPCR analysis

Gene	Forward	Reverse	Accession	Amplicon size (bp)
IL1β	CAGTCTTCAGTGCTCAGGTTTCTG	CATTGCCGCTGCAGTAAGT	XM_001495926.4	84
IL1RN	GCCTGTGTCAAGTCTGGTGA	ACTCTTTGGGCTTGTTGGTG	XM_005599766.3	217
IL6	GGATGCTTCCAATCTGGGTTCAAT	TCCGAAAGACCAGTGATGATTTT	NM_001082496.2	65
PLAC8	GTTTCATGTGCCTTGCGTTGT	ATATCGGGTCCGGTAGAGGG	XM_003364673.4	98
SAA1	CCTGGGCTGCTAAAGTCATC	AGGCCATGAGGTCTGAAGTG	XM_023646155.1	169
INHBA	CCTCCTCTTCCTTCTTCTTCTTCTT	GCAAGAGCTCCCTGGATATT	XM_023638729.1	99
GUSB	GGGATTCGCACTGTGGCTGTCA	CCAGTCAAAGCCCTTCCCTCGGA	XM_001493514	117
ACTB	CGACATCCGTAAGGACCTGT	CAGGGCTGTGATCTCCTTCT	NM_001081838	100

Primers were generated using Primer-BLAST (NCBI).
Key: RT-qPCR, Real time quantitative polymerase chain reaction; NCBI, National Center for Biotechnology Information; IL1β, Interleukin 1β; IL1RN, Interleukin 1 Receptor Antagonist; IL6, Interleukin 6; PLAC8, Placenta-specific 8; SAA1, Serum Amyloid A1; INHBA, Inhibin Subunit Beta A; ACTB, actin beta; GUSB, Glucuronidase Beta.

ISSN: 1297-9716