Figure 8

RT-qPCR validation of six selected differentially expressed genes from RNA-sequencing results. Expression of each mRNA was determined by qRT-PCR, normalized to ACTB and GUSB, expressed as −∆CT. Data are presented as a dot plot, and the middle horizontal line represents the mean while error bars represent the standard error of the mean (SEM). Asterisks indicate the presence of a significant difference between groups (*P < 0.05; **P < 0.01, *** P < 0.001). Dagger (†) indicates the presence of a trend (0.1 > P ≥ 0.05). Pearson's correlation coefficient (r) between mRNA expression results from RT-qPCR (−ΔCT) and the RNA-sequencing (FPKM) is represented for each transcript. Key; IL1β, Interleukin 1β; IL1RN, Interleukin 1 Receptor Antagonist; IL6, Interleukin 6; PLAC8, Placenta-specific 8; SAA1, Serum Amyloid A1; INHBA, Inhibin Subunit Beta A; ACTB, actin beta; GUSB, Glucuronidase Beta.